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1.
山羊子宫内肽能神经分布及妊娠时的变化 总被引:4,自引:1,他引:3
采用免疫组化方法,研究了山羊子宫内含P物质(SP)和含血管活性肠肽(VIP)神经的分布。结果,妊娠及未妊娠山羊子宫颈内有粗细不等的神经束行经于外膜和肌层中,神经分支形成丛状分布于血管壁,子宫颈部未见SP神经元胞体及VIP神经元胞体;未妊娠山羊子宫角内SP神经和VIP神经均呈丛状围绕血管并分布于血管壁;妊娠中期的孕角和非孕角均有胎盘形成,除胎盘内无神经分布外,SP神经及VIP神经同样分支形成丛状分布于血管壁。结果提示,山羊子宫内SP神经和VIP神经主要支配子宫内血管,妊娠时除胎盘内无神经分布外,SP神经及VIP神经的分布无明显变化。 相似文献
2.
3.
鸣禽鸟的发声中枢及其神经传导通路的研究曾庆华,赵玲,姜立嘉(东北师范大学生物系)鸣禽鸟之所以能发出婉动听的鸣叫,不仅仅是依赖于外围发声器(鸣管)来完成,而是经过多级水平的相关中枢组成的机能系统密切配合统一调节方能完成的。这个机能系统称它为各级发声中枢... 相似文献
4.
Steven B. Symington 《Pesticide biochemistry and physiology》2005,82(1):1-15
Isolated presynaptic nerve terminals prepared from whole rat brain were used to evaluate the action of deltamethrin on voltage-sensitive calcium channels by measuring calcium influx and endogenous glutamate release. Deltamethrin-enhanced K+-stimulated calcium influx and subsequent Ca2+-dependent glutamate release. The effect of deltamethrin was concentration-dependent, stereospecific, blocked by ω-conotoxin MVIIC but unaltered in the presence of tetrodotoxin. These results suggest that N-type voltage-sensitive calcium channels are a site of action at the presynaptic nerve terminal. Electrophysiological studies were carried out using rat brain Cav2.2 and β3 subunits coexpressed in Xenopus oocytes to validate such action. Deltamethrin reduced barium peak current in a concentraion-dependent and stereospecific manner, increased the rate of activation, and prolonged the inactivation rate of this channel. These experiments support the conclusion that N-type voltage-sensitive calcium channel operation is altered by deltamethrin. 相似文献
5.
运用免疫组织化学超敏 SP法对山羊胎儿脊髓发育中神经生长因子 (nerve growth factor,NGF)及其高亲和力受体 Tr KA的表达及其功能进行了系统的研究和探讨。结果显示 ,山羊胎儿脊髓灰质中存在 NGF及其受体 Tr KA,于 6周龄胚就可检测到 ,随胚龄增加 ,其表达范围及免疫反应着色程度逐渐增强。 NGF主要分布于腹角和背角的神经细胞 ,反应产物主要定位于胞质和突起 ;Tr KA的分布主要以腹角及胶状质为主 ,反应产物主要定位于胞核 ,后期胞质及突起也可见到阳性反应。在山羊胎儿脊髓白质中也可观察到 NGF及 Tr KA免疫阳性反应 ,其发育后期更为显著 ,阳性反应主要分布于神经胶质细胞核、神经纤维的轴索及雪旺氏细胞。结果提示 ,NGF不仅对交感和感觉神经元的发育起作用 ,而且还与腹角运动神经元的发育有关 相似文献
6.
通过大鼠急性毒性试验、大鼠脊髓损伤运动功能试验和大鼠脊髓含水量试验,对小美牛肝菌生物碱抑制运动神经系统的原理进行研究。大鼠急性毒性试验结果表明,小美牛肝菌生物碱含有一定的毒性,对机体组织的运动神经系统有一定的影响;大鼠脊髓损伤运动功能试验结果表明,小美牛肝菌所含的生物碱物质能够促进大鼠脊髓损伤恢复,降低大鼠脊髓神经损伤;大鼠脊髓含水量试验结果表明,小美牛肝菌生物碱具有消炎抗氧化作用,能够降低过氧化物对神经细胞的损害。 相似文献
7.
AIM: To investigate the effect of electroacupuncture treatment on microglia phenotype in Beagle dogs with abducens nerve injury. METHODS: Healthy adult Beagle dogs were randomly divided into 4 groups:normal control group, sham operation group, injury group and electroacupuncture treatment group, with 5 dogs in each group. In sham operation group, only the isolated nerves were exposed. In injury group, the dogs were restrained when electroacupuncture was performed in electroacupuncture treatment group. In electroacupuncture treatment group, electroacupuncture was performed on the base of dog nerve injury. The experiment was continued for 2 weeks. Western blot was used to detect the expression levels of M1 and M2 markers in the microglia. RESULTS: The expression of inducible nitric oxide synthase (iNOS) in injury group was significantly higher than that in control group (P<0.05). In electroacupuncture treatment group, the expression of iNOS was significantly lower than that in injury group (P<0.05). The expression of interleukin-1β (IL-1β) in injury group and electroacupuncture treatment group was higher than that in control group and that in electroacupuncture treatment group was significantly lower than that in injury group (P<0.05). The expression of arginase in injury group and electroacupuncture treatment group was higher than that in control group (P<0.05), and that in electroacupuncture treatment group was higher than that in injury group (P<0.05). Meanwhile, the expression of brain-derived neurotrophic factor (BDNF) in injury group and electroacupuncture treatment group was increased compared with control group (P<0.05). But the expression of BDNF in electroacupuncture treatment group was higher than that in injury group (P<0.05).CONCLUSION: After abducens nerve injury, electroacupuncture treatment reduces the expression levels of M1 marker proteins in microglia and increases the expression levels of M2 marker proteins. 相似文献
8.
Flavio A.V. Freitag Dorli da S. Amora Eloisa Muehlbauer Peterson T. Dornbusch Marcello Machado Fabiano Montiani-Ferreira Edison L. Prisco Farias Alexander Valverde Juan Carlos Duque Moreno 《Veterinary anaesthesia and analgesia》2021,48(4):596-602
ObjectiveTo describe and evaluate an ultrasound-guided modified subcostal approach for the transversus abdominis plane (TAP) block in horse cadavers in lateral or dorsal recumbency.Study designProspective, experimental cadaveric study.AnimalsStudy of one preserved foal and eight fresh adult horse cadavers.MethodsThe lateral and ventral abdominal wall of a preserved cadaver was dissected to identify the muscles and nerves. A unilateral standard TAP block technique was performed (60 mL of methylene blue dye–bupivacaine) on a fresh cadaver in right lateral recumbency. A modified subcostal technique was performed on the opposite side using a linear ultrasound transducer and in-plane approach. Injection points (two 30 mL dye) were at the level of the TAP (between the rectus abdominis and transversus abdominis muscles and ventral to the cutaneous trunci muscle) perpendicular to: 1) the mid-point between the xiphoid cartilage and umbilical scar; and 2) at a point between the caudal and middle thirds of the abdomen measured from the first injection point to the umbilical scar. The modified subcostal approach was performed in seven additional cadavers in both hemiabdomens, with three cadavers in lateral and four cadavers in dorsal recumbency. Ultrasound guidance was used with all injections.ResultsThe standard approach stained the sixteenth to eighteenth thoracic nerves (T16–T18). The modified subcostal approach performed in lateral recumbency provided greater spread (T9–T17) than dorsal recumbency (T12–T18) (p = 0.016).Conclusions and clinical relevanceThe modified subcostal TAP approach resulted in extensive staining exceeding the standard approach. The nerves stained are consistent with production of ventral abdominal wall anesthesia in horses. Clinical studies are needed to verify these findings. 相似文献
9.
Vincent Marolf Claudia Spadavecchia Nicole Müller Charlotte Sandersen Helene Rohrbach 《Veterinary anaesthesia and analgesia》2021,48(3):398-406
ObjectiveTo determine the intraoperative and early postoperative opioid requirement after ultrasound-guided sciatic and/or femoral nerve block or epidural anaesthesia in dogs undergoing tibial plateau levelling osteotomy (TPLO).Study designProspective, masked, pilot, randomized, clinical trial.AnimalsA total of 40 client-owned dogs undergoing TPLO.MethodsEach dog was randomly assigned to group SF (combined sciatic and femoral nerve block), group S (sciatic nerve block), group F (femoral nerve block) or group E (epidural anaesthesia). A total of 0.3 mL kg–1 of ropivacaine 0.5% was administered to each nerve or in the epidural space. Intraoperatively, fentanyl (2 μg kg–1) was administered intravenously when heart rate, mean arterial pressure or respiratory rate increased by >30% compared with baseline values. Postoperatively, a visual analogue scale (VAS) and a modified German version of the French pain scale (4AVet) were used to assess pain every 30 minutes for 150 minutes and again once the morning after surgery. Methadone (0.1 mg kg–1) was administered intravenously if the VAS was ≥ 4 cm [maximal value 10 cm; median (interquartile range)] or the composite pain score was ≥5 [maximal value 15; median (interquartile range)]. Significance was defined as p ≤ 0.05.ResultsGroups SF and E required less total intraoperative and early postoperative opioid doses compared with groups S and F (p = 0.031). No dogs in group SF had a block failure or required postoperative methadone. A reduced methadone requirement was found in group SF compared with all the other groups up to 150 minutes after recovery (p = 0.041).Conclusions and clinical relevanceCombined sciatic and femoral nerve block and epidural anaesthesia lead to less cumulative consumption of perioperative opioids than single nerve blockade. Sciatic or femoral nerve block alone might be insufficient to control nociception and early postoperative pain in dogs undergoing TPLO. 相似文献
10.
Alexandra B. Kalamaras Turi K. Aarnes Sarah A. Moore Stephen C. Jones Carolina Ricco Pereira Juan Peng Nina R. Kieves 《Veterinary anaesthesia and analgesia》2021,48(3):415-421
ObjectiveTo compare the quality of postoperative analgesia and sedation after preoperative saphenous and sciatic nerve blockade, preoperative lumbosacral epidural injection and perioperative intravenous (IV) morphine, lidocaine and ketamine infusions in dogs undergoing stifle arthroscopy and tibial plateau leveling osteotomy (TPLO) under general anesthesia.Study designProspective, blinded, randomized, clinical comparison study.AnimalsA total of 45 dogs weighing 33.9 (15.9–56.7) kg and aged 5.2 (1.0–12.0) years, mean (range), undergoing elective unilateral TPLO for spontaneous cranial cruciate ligament rupture.MethodsClient-owned dogs were enrolled. Dogs were randomly assigned to one of three groups: group MLK, perioperative IV morphine, lidocaine and ketamine infusion; group EPID, lumbosacral epidural with ropivacaine and morphine; or group SSNB, saphenous and sciatic nerve blockade with ropivacaine. Routine stifle arthroscopy followed by TPLO surgery was performed. Sedation and pain scores were assessed at 0, 2, 4, 8 and 24 hours following extubation. Rescue analgesia was administered as prescribed by Glasgow composite pain score–short form score >5.ResultsSedation scores for MLK were higher than EPID and SSNB. Pain scores for SSNB were lower than those for EPID and MLK. No significant differences were found in anesthesia duration or surgery duration among groups. No dogs required rescue analgesia.Conclusions and clinical relevanceAlthough analgesia was adequate in all groups, the best combination of analgesia without increased sedation was recorded for SSNB. 相似文献